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rabbit anti p mtor  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti p mtor
    Rabbit Anti P Mtor, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p+mtor/pm41763584-177-48-77
    Average 86 stars, based on 1 article reviews
    rabbit anti p mtor - by Bioz Stars, 2026-09
    86/100 stars

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    Incubation:

    Article Title: Edaravone dexborneol promotes oligodendrocyte precursor cell proliferation and differentiation to repair white matter and improve long-term outcomes after ischemic stroke.
    Article Snippet: Then, 25 μg of protein was subjected to electrophoresis using 10–12% SDS polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (cat. no. 10600021; GE Healthcare). .. The membranes were blocked with 10% non-fat milk in Tween/Tris-buffered salt solution (20 mM Tris-Cl pH 7.5, 0.15 M NaCl, 0.05% Tween-20) for 1 h, then incubated overnight at 4̊C with rabbit anti-Akt (cat. no. 4691), rabbit anti-phosphorylated (p-)Akt (Thr308; cat. no. 13038), rabbit anti-mTOR (cat. no. 2983), rabbit anti-p-mTOR (Ser2481; cat. no. 2974), rabbit anti-S6 ribosomal protein (cat. no. 2217), rabbit anti-p-S6 (Ser235/236; cat. no. 4858) and rabbit anti-GAPDH (cat. no. 2118) primary antibodies (all from CST Biological Reagents Co., Ltd. and used at a dilution of 1:1000). .. After washing for 30 min, the PVDF membranes were incubated with the horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody (Abcam; cat. no. ab6721; 1:10,000) at room temperature for 1.5 h. Reactivity was visualized using an enhanced chemiluminescence (ECL) kit (MilliporeSigma) and captured with a Bio-Rad ECL detection system.

    Article Title: Targeting PI3K-mTOR signaling in the anterior cingulate cortex improves emotional behavior, and locomotor activity in rats with bone cancer pain
    Article Snippet: .. Next, the membrane was incubated overnight with the following primary antibodies at 4 °C: rabbit anti-p-mTOR (1:500, ab8440, Cell Signaling, USA), rabbit anti-p-P70s6k (1:500, ab60948, Cell Signaling), mouse anti-p-PI3K (1:500, ab182651, GENETEX), rabbit anti-p-4E-BP (1:500, ab2606, Cell Signaling), and rabbit anti-β-tubulin (1:500, CB65591556, Multi Sciences, China). .. Following the primary antibody incubation, goat anti-rabbit and anti-mouse IgG (1:5000, Cell Signaling) were added as secondary antibodies, respectively, and incubated at room temperature for 2 hours.

    Immunohistochemistry:

    Article Title: Vitamin D Regulates Olfactory Function via Dual Transcriptional and mTOR-Dependent Translational Control of Synaptic Proteins.
    Article Snippet: Capillary cartridges (Protein Simple, SM-W004-1), anti-rabbit detectionmodule chemiluminescence (Protein Simple DM-001), anti-mouse detection module chemiluminescence (Protein Simple DM-002), and EZ standard pack (Protein Simple PS-ST01EZ-8) were used according to the manufacturer’s instructions. .. The following antibodies were used (if notmentioned in the immunohistochemistry section): mouse anti-β-actin (1:200, Novus, NB600501), rabbit anti-AKT (1:200, Cell Signaling Technology, 4691), rabbit antip-AKT (1:20, Cell Signaling Technology, 4060), rabbit anti-eIF4E (1:100, Cell Signaling Technology, 2067), rabbit anti-eIF4EBP1 (1:100, Cell Signaling Technology, 9644), rabbit anti-p-eIF4EBP1 (1:200, Cell Signaling Technology, 2855), rabbit anti-eIF4EBP2 (1:20, CST, 2845), rabbit anti-GAPDH (1:5000, Cell Signaling Technology, 5174), mouse anti-gephyrin (1:4000, Synaptic Systems, 147 111), rabbit anti-GFP (1:1000; Invitrogen A-11122), rabbit anti-mGluR1 (1:500, Cell Signaling Technology, 12 551), rabbit antiMNK (1:100, Cell Signaling Technology, 2195), rabbit anti-p-MNK (1:20, Cell Signaling Technology, 2111), mouse anti-mTOR (1:1000, CST, 4517), rabbit anti-p-mTOR (1:50, CST, 5536), rabbit anti-PI3K (1:3000, Cell Signaling Technology, 4249), rabbit anti-p-PI3K (1:20, Cell Signaling Technology, 17 366), rabbit anti-PSD95 (1:100, Cell Signaling Technology, 3409), rabbit anti-Rps6 (1:100, Cell Signaling Technology, 2217), rabbit anti-S6K (1:200, CST, 33 475), rabbit anti-p-S6K (1:50, CST, 9234), mouse antisynapsin1 (1:7000, Synaptic Systems, 106 011), mouse anti-VGAT (1:20, Synaptic Systems, 131 011),mouse anti-vGlut1 (1:7000, Synaptic Systems, 135 011), and rabbit anti-vGlut2 (1:100, Cell Signaling Technology, 16 066). .. RNA Library Construction and Sequencing (Bulk RNA-seq): Total RNA of OBs was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen, Hilden, Germany).

    Membrane:

    Article Title: Targeting PI3K-mTOR signaling in the anterior cingulate cortex improves emotional behavior, and locomotor activity in rats with bone cancer pain
    Article Snippet: .. Next, the membrane was incubated overnight with the following primary antibodies at 4 °C: rabbit anti-p-mTOR (1:500, ab8440, Cell Signaling, USA), rabbit anti-p-P70s6k (1:500, ab60948, Cell Signaling), mouse anti-p-PI3K (1:500, ab182651, GENETEX), rabbit anti-p-4E-BP (1:500, ab2606, Cell Signaling), and rabbit anti-β-tubulin (1:500, CB65591556, Multi Sciences, China). .. Following the primary antibody incubation, goat anti-rabbit and anti-mouse IgG (1:5000, Cell Signaling) were added as secondary antibodies, respectively, and incubated at room temperature for 2 hours.



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